Brucellosis Antibody Elisa Kit For Bovine, Sheep, Pig And Dog Etc.
The Brucellosis antibody ELISA kit is used to test Brucellosis antibody in serum of bovine, goat, sheep, pig and dog etc.
This kit use competitive ELISA method, Brucellosis antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is Brucellosis specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-Brucellosis virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
Brucellosis Antibody ELISA Kit – Bovine, Ovine, Porcine, and Canine (96-Well Plate)
The Brucellosis Antibody ELISA Kit is a species-adapted, indirect enzyme-linked immunosorbent assay (ELISA) for the qualitative detection of antibodies directed against Brucella spp. (primarily B. abortus, B. melitensis, and B. suis) in serum or plasma samples from cattle (bovine), sheep (ovine), pigs (porcine), and dogs (canine). This assay supports epidemiological surveillance, diagnosis, and confirmation of Brucella infection status in veterinary populations.
Key Specifications:
- Format: 96-well microtiter plate, pre-coated with inactivated Brucella antigen (smooth lipopolysaccharide).
- Detection Method: Species-specific HRP-conjugated secondary antibody.
- Readout: Colorimetric measurement at 450 nm.
- Assay Type: Indirect ELISA.
- Sensitivity: >95% (dependent on host species and disease stage).
- Specificity: >98% (validated against non-Brucella pathogens with no significant cross-reactivity).
- Sample Volume: 50 µL per well.
- Assay Time: ~2 hours total.
- Sample Types: Serum, plasma (EDTA or heparinized).
Principle:
Wells are pre-coated with purified Brucella LPS antigen. Serum samples are added to the wells, allowing specific anti-Brucella antibodies—if present—to bind the antigen. After washing, an HRP-conjugated anti-species IgG (adapted for each host species) is added. Following incubation and further washing, a TMB substrate is introduced, resulting in a measurable color change. The reaction is stopped with acid and optical density is measured at 450 nm. Samples with absorbance values above a predetermined cutoff are considered positive for Brucella-specific antibodies.
Kit Validation:
- Validated in bovine, ovine, porcine, and canine sera.
- Internal and external proficiency-tested reference standards included.
- Lot-to-lot reproducibility controlled.
- Coefficient of variation (CV): intra-assay <10%, inter-assay <15%.
Applications:
- Veterinary diagnostics for brucellosis control programs.
- Confirmation of brucellosis vaccination status and seroconversion.
- Surveillance in livestock populations and zoonotic risk assessment in working dogs.
Kit Contents:
- Pre-coated 96-well ELISA microplate
- Positive and negative control sera (species-specific)
- Sample Diluent and Wash Buffer (concentrate)
- Species-adapted HRP-conjugate
- TMB Substrate Solution
- Stop Solution (0.5 M H₂SO₄)
- Detailed instruction manual and plate sealers
Storage and Stability:
- Store at 2–8°C; do not freeze.
- Shelf life: 12 months from production date.
- Opened reagents remain stable if properly stored and handled according to the kit protocol.
